Figure 3

DSB response triggers p53 signaling under genomic stress. A. Immunofluorescence staining showing expression of RAD50, NPM1, XRCC5, H2AX-γ and p53 at steady state and 4 μM cisplatin treated control siRNA, siRAD50, siNPM1 and siXRCC5 A4-T cells. B. Quantitation of ATM, H2AX-γ, p53, p21, CDK1, Cyclin D1 and Bcl-2 protein expressions in untreated and cisplatin treated A4-T, control siRNA, siRAD50, siNPM1 and siXRCC5 A4-T cells. C. In-Situ fluorescein cell death (TUNEL) analysis showing nuclear fragmentation (red foci) in untreated and cisplatin treated control siRNA, siRAD50, siNPM1 and siXRCC5 A4-T cells (Red color- pseudo color instead of green). D. Quantitative analysis of TUNEL assay showing percentage apoptotic populations in complete set of untreated and cisplatin treated A4-T, control siRNA, siRAD50, siNPM1 and siXRCC5 cells. Scale bar is 12 μm. Data shown as mean ± SE of triplicate experiments. *p <0.05; **p <0.01; ***p <0.001.