Figure 2From: Targeted gene silencing using a follicle-stimulating hormone peptide-conjugated nanoparticle system improves its specificity and efficacy in ovarian clear cell carcinoma in vitro Expression of gro-α mRNA and protein after treatment with siRNA targeted to gro-α. (A) Detection of gro-α mRNA expression by qRT-PCR. (B) Detection of gro-α protein expression in cell supernatants by ELISA. ES-2 cells were treated with one of four siRNA sequences along with DharmaFECT transfection reagent for 4 h. Gro-α mRNA and gro-α protein secreted in cell supernatants were quantified 24 h or 48 h after treatment.Back to article page