Figure 5

Effect of over expression of members of miR-200 family in HEY cells on cisplatin sensitivity. (A) IC₅₀ values are presented as mean ± SEM of at least 3 independent experiments with 3 replicated wells per drug concentration. Statistical analysis was performed using ANOVA and Tukey’s multiple comparison test as a post-hoc test. Asterisks represent significant differences from the negative control (nc-miR) group, *P ≤0.05, **P ≤0.005. (B) Qualitative evaluation of cell viability in presence of (i) 20 μM and (ii) 0 μM cisplatin in HEY cells alone or transfected with different members of miR-200 family or nc-miR (crystal violet staining; Scale bars, 100 μm).