Figure 3

Versican contributes to EOC spheroid disaggregation. (A) Unmodified SKOV-3, SKOV-3 stably transfected with scrambled shRNA and VCAN shRNA (2 different clones), and SKOV-3 transiently transfected with control or VCAN siRNA, as indicated, were labeled with fluorescent DiI and used in spheroid formation assay. Measurements of the total area taken by spheroids were taken at 0 and 24 h time points and used for calculation of the area fold change ratios. Presented is an average of at least 3 independent experiments; error bars represent standard deviation; *p < 0.05, comparisons were made between experimental (VCANsi, VCANsh) and control (SKOV-3, Scr sh, Ctrl si) groups. (B) A typical image of disaggregating SKOV-3 and SKOV-3 VCAN clone 5 spheroids taken at 0 and 24 h. Bar, 100 micron. (C) Unmodified SKOV-3, SKOV-3 stably transfected with scrambled shRNA and VCAN shRNA (2 different clones), and SKOV-3 transiently transfected with control or VCAN siRNA, as indicated, were used in spheroid formation assay. Presented is an average of at least 3 independent experiments; error bars represent standard deviation; *p < 0.05, comparisons were made between experimental (VCANsi, VCANsh) and control (SKOV-3, Scr sh, Ctrl si) groups.