Figure 4
The electron micrograph of granulosa cells of a secondary follicle. A and B) vitrified ovaries in single cryoprotectant, C) vitrified ovaries in double cryoprotectant, showing arrangement of mithochondria m, numerous lipid droplets Lp, vacuole V and atypical or irregularly shaped mithochondria with longitudinally oriented cristae were found in CV1-4 groups or single cryoprotectant, TEM: ×10000. B; numerous spherical mithochondria with continuous membranes and normal cristae were obserced in CV6, 7 groups or double cryoprotectant, A; TEM: ×4646, B and C; TEM: ×10000.