Fig. 1

Photomicrographs of histological sections of minipig ovaries. Follicles in all stages of development was detected across all groups (bar = 50 μm; HE staining, 100×). a - animals that only underwent bilateral laparoscopic oophorectomy, the ovarian tissue of which was removed during oophorectomy for use as the control (GI); b - animals that underwent bilateral oophorectomy and immediate autologous transplantation of fresh ovarian tissue into the subcutaneous tissue (GII); c - animals that underwent bilateral oophorectomy and immediate autologous transplantation of fresh ovarian tissue into the peri-infundibular intraperitoneal site (GIII); d - animals that underwent bilateral oophorectomy and subsequent autologous transplantation of cryopreserved ovarian tissue into the subcutaneous tissue (GIV); e - animals that underwent bilateral oophorectomy and subsequent autologous transplantation of cryopreserved ovarian tissue into the peri-infundibular intraperitoneal ovarian region (GV)