Fig. 3

Silencing of PKP3 stimulates the proliferation of ovarian granulosa cells and represses their apoptosis. A, mRNA expression of PKP3 determined by RT-qPCR in ovarian granulosa cells treated with sh-PKP3-1 or sh-PKP3-2. B-C, Viability (B) and apoptosis (C) of ovarian granulosa cells with PKP3 knockdown measured by CCK-8 assay and flow cytometry. D, Expression of Bcl-2, Bax, and cleaved caspase-3 in ovarian granulosa cells with PKP3 knockdown determined by Western blot analysis. E, Cell cycle distribution of ovarian granulosa cells with PKP3 knockdown measured by flow cytometry. * p < 0.05, ** p < 0.01. The cell experiment was conducted three times independently