Fig. 4

PKP3 enhances the expression of ERCC1 by activating the MAPK pathway. A, CFDTW component-potential target-pathway network diagram. B, Venn diagram of the differentially expressed genes in the PCOS samples in the GSE34526 dataset and CFDTW potential targets. C, An expression heat map of the intersection genes in the GSE34526 dataset. D, A box plot of ERCC1 gene expression in the GSE34526 dataset. Blue represents control samples (n = 3) and red represents PCOS samples (n = 7). E, ERCC1 protein expression and the extent of ERK1/2 and p38 phosphorylation in ovarian granulosa cells with PKP3 knockdown determined by Western blot analysis. F, ERCC1 protein expression and the extent of ERK1/2 and p38 phosphorylation in ovarian granulosa cells from PCOS patients with PDS or those treated with CFDTW determined by Western blot analysis. G, ERCC1 protein expression and the extent of ERK1/2 and p38 phosphorylation in ovarian tissues of PCOS rats (n = 8) or those treated with CFDTW (n = 8) determined by Western blot analysis. * p < 0.05. The cell experiment was conducted three times independently