Fig. 5

CFDTW stimulates the proliferation of ovarian granulosa cells and represses their apoptosis by regulating the PKP3/MAPK/ERCC1 axis. A, ERCC1 mRNA expression in ovarian granulosa cells treated with sh-ERCC1-1 or sh-ERCC1-2 determined by RT-qPCR. Ovarian granulosa cells were treated with Vector + sh-NC + CFDTW, PKP3 + sh-NC + CFDTW or PKP3 + sh-ERCC1 + CFDTW. B, ERCC1 protein expression and the extent of ERK1/2 and p38 phosphorylation in ovarian granulosa cells determined by Western blot analysis. C-D, Viability (C) and apoptosis (D) of ovarian granulosa cells measured by CCK-8 assay and flow cytometry. E, Expression of Bcl-2, Bax, and cleaved caspase-3 in ovarian granulosa cells determined by Western blot analysis. F, Cell cycle distribution of ovarian granulosa cells measured by flow cytometry. * p < 0.05. The cell experiment was conducted three times independently