Fig. 1

FSH combined with S1P protects ovaries from vitrification-induced damage

(A) HE-stained sections of vitrified-thawed ovaries (Scale bar: 200 μm). (B) Follicle count at each stage (PF: primordial follicles; P: primary follicles; S: secondary follicles; A: antral follicles; AF: atretic follicles). (C-D) TUNEL staining to detect apoptosis of vitrified-thawed ovaries (Scale bar: 200 μm) and statistical analysis of apoptotic rate. (E-F) Western blot analysis of the expression of Cleaved Caspase-3 in vitrified-thawed ovaries and its quantification. (G-H) Western blot analysis of the expression of Bcl-2 and Bax in vitrified-thawed ovaries and quantification of Bcl-2/Bax ratio. (*P < 0.05; **P < 0.01)