Fig. 2

FSH combined with S1P protects the ovaries from ischemia-hypoxia injury at 24 h after transplantation

(A) HE-stained sections of ovaries at 24 h after transplantation (Scale bar: 200 μm). (B) Follicle count at each stage (PF: primordial follicles; P: primary follicles; S: secondary follicles; A: antral follicles; AF: atretic follicles). (C-D) TUNEL staining to detect ovarian apoptosis at 24 h after transplantation (Scale bar: 200 μm) and statistical analysis of apoptotic rate. (E-F) Western blot analysis of the expression of Cleaved Caspase-3 of ovaries at 24 h after transplantation and its quantification. (G-H) Western blot analysis of the expression of Bcl-2 and Bax of ovaries at 24 h after transplantation and quantification of Bcl-2/Bax ratio. (I-L)Western blot analysis of the expression of LC3B, Beclin-1 and P62 of ovaries at 24 h after transplantation and its quantification. (*P < 0.05; **P < 0.01)