Fig. 3
FSH combined with S1P promotes follicle survival and angiogenesis of ovaries at 7 days after transplantation
(A) HE-stained sections of ovaries at 7 days after transplantation (Scale bar: 200 μm). (B) Follicle count at each stage (PF: primordial follicles; P: primary follicles; S: secondary follicles; A: antral follicles; AF: atretic follicles). (C-D) Immunohistochemical analysis of the expression of Cx37, Cx43, and VEGF, and statistical analysis of the respective positive areas (Scale bar:100 μm). (E) The graft angiogenesis is visualized by injecting the mice with 2 MDa FITC-dextran. The merged images of CD31 (red) and FITC-dextran angiography (green) were obtained by the confocal laser scanning microscope (Scale bar: 200 μm; high magnification: 100 μm). (F) Percentage of CD31-positive area of ovaries to the total area at 7 days after transplantation. (G) Percentage of CD31-positive area of ovaries to FITC-dextran positive area at 7 days after transplantation. (H) Results of ELISA showing the serum levels of E2 of mice at 7 days after transplantation. (*P < 0.05; **P < 0.01)