Fig. 4

Molecular mechanism of FSH combined with S1P intervention to protect follicle survival in transplanted ovaries

(A-B) Western blot analysis of the expression of Cleaved Caspase-3 in KGN cells treated with ischemia-hypoxia and its quantification. (C-D) Western blot analysis of the expression of Bcl-2 and Bax in KGN cells treated with ischemia-hypoxia and quantification Bcl-2/Bax ratio. (E) After KGN cells were transfected with GFP-MAP1LC3B plasmid, the production of autophagosomes in the cells was observed by the confocal laser scanning microscope (Scale bar: 10 μm). (F) Quantification of GFP-MAP1LC3B puncta per cell. (G-J) Western blot analysis of the expression of LC3B、Beclin-1 and P62 in KGN cells treated with ischemia-hypoxia and its quantification. (K-M) Western blot analysis of the expression of p-AKT and p-mTOR in KGN cells treated with ischemia-hypoxia and its quantification. (N-O) Western blot analysis of the expression of p-mTOR in KGN cells treated with the mTOR inhibitor Rapamycin. (P-Q) Western blot analysis of the expression of LC3B in KGN cells treated with Rapamycin. (*P < 0.05; **P < 0.01)