Fig. 3
From: MUC16 stimulates neutrophils to an inflammatory and immunosuppressive phenotype in ovarian cancer
MUC16 altered the immune phenotype of neutrophils similar to ovarian cancer organoids. (A) Immunofluorescence staining showed MUC16 adhesion to Siglec-9+ neutrophils after stimulation by ovarian cancer organoids. NC: negative control group without ovarian cancer organoid stimulation; OR: ovarian cancer organoid stimulation group. Bar = 50 μm. (B) The proportion of CD11b+, CD66b+, ICAM-1+,CXCR4+ neutrophils in the MUC16 stimulation group (MUC16) and control group (NC). The upper panel shows the representative flow cytometry results; the lower panel shows the experimental results of 5 cases of neutrophils from different patients. Paired t-test. (C-D) The expression of function-related factors in the MUC16 stimulation group compared to the control group determined by qPCR (three independent replicate experiments of neutrophils derived from different patients). Error Bar = Mean ± SEM. (E) Detection of ROS by flow cytometry with DCFH-DA stimulation. Representative results are shown on the left. Blank: blank control group without DCFH-DA; NC: negative control group; MUC16: MUC16 treatment group. N = 5, paired t-test, *p < 0.05. (F) The flow cytometry results showed the proportion of Siglec-9+ neutrophils in the MUC16-treated group (MUC16) and the control group (NC). The left panel shows the representative flow cytometry results; the right panel shows the experimental results of 5 cases of neutrophils from different patients. Paired T-test, *p < 0.05