Fig. 4

MUC16-stimulated neutrophils showed an inflammatory and immunosuppressive phenotype. (A) Circos of GO BP enrichment analysis of differentially expressed genes in the MUC16 treatment group compared with the control group. (B) Results of GSEA analysis according to GO BP in the MUC16-treated group compared to the control group. (C) Circos of Hallmarks enrichment analysis of differentially expressed genes in the MUC16 treatment group compared to the control group. (D) Results of GSEA analysis according to Hallmarks in the MUC16 treated group compared to the control group. (E) Volcano plot of differentially expressed genes (|log2FC|>2, p < 0.05) in the MUC16-treated group compared to the control group. (F) Expression of chemokines and receptors in the MUC16-treated group (MUC16) and the control group (NC). Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001. (G) The expression of immunoregulatory factors in the MUC16 treatment group (MUC16) and the control group (NC). Student’s t-test, *p < 0.05, **p < 0.01. (H) The levels of IL-6, IL-8, IL-10, and TNF-α secreted by neutrophils in the MUC16-treated group (MUC16) and the control group (NC). Student’s t-test, ns: none significance, *p < 0.05, **p < 0.01. Error Bar = Mean ± SEM. (I) Effect of neutrophil supernatant treatment on NK-92 killing of OVCAR3. N1/2/3 are the culture supernatants of neutrophils from different patients, respectively. Student’s t-test, n.s. none significance, *p < 0.05, **p < 0.01. Error Bar = Mean ± SEM.