Fig. 6

Effects of GH treatment on proliferation and apoptosis of lamb GCs. The cells were cultured in medium containing 100 µg/ml GH for 12 h and then were used for detection. a The representative images depicting EdU positive GCs (red) (left, scale bar = 20 μm) and the number of EdU positive cells of total GCs (right). Arrows point out EdU positive cells. b The relative mRNA levels of cell proliferation related genes detected by qRT-RCR. c Co-immunostaining of BAX (green) and BCL-2 (red) in lamb GCs (left, scale bar = 20 μm) and the quantification of BAX/BCL-2 levels in lamb GCs detected by fluorescence intensity (right). d The relative mRNA levels of cell apoptosis related genes detected by qRT-RCR. e-f The cell apoptosis was staining for PI/Annexin V and detected by flow cytometry. *P<0.05; **P<0.01; ***P<0.001