Fig. 6

ASP reverses DHEA induced decrease of PRKCA in PCOS granulosa cells. A-C qRT-PCR and Western blot were performed to detect the expression of PRKCA in KGN cells. β-actin was used as a normalization standard. D Estrus status of mice in the DHEA and control groups (n = 6 per group). E ELISA assay for T and E2 in mouse serum. F Histological staining of ovaries from DHEA and control mice. G qRT-PCR was performed to detect the expression of PRKCA in PCOS mouse models