Fig. 1

Omega-3 PUFA supplementation attenuates metabolic and endocrine disorders in DHEA-induced PCOS mice. A Experimental design. B Body weights (n = 6). C-G The serum levels of FSH (C), LH (D), E2 (E), T (F) and LH/FSH (G) were measured by ELISA (n = 6). H Fasting blood glucose (n = 10). I GTT (n = 10). J ITT (n = 10), * represents the control group compared to the PCOS group, and # represents PCOS mice compared to the omega-3 group. The data are shown as the mean ± SEM with individual values. Statistical analyses were carried out using one-way ANOVA followed by Dunnett’s multiple comparisons test (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). FSH, follicle-stimulating hormone; LH, luteinizing hormone; E2, estradiol 2; T, testosterone; GTT, glucose tolerance test; ITT, insulin tolerance test