Fig. 3

Omega-3 PUFA treatment has an obvious effect on the α-diversity but not β-diversity of the gut microbiota in PCOS mice. The microbiota composition of control, PCOS and omega-3 mice was analyzed by 16 S rDNA pyrosequencing (n = 3 for each group). A Shannon curve. B Sob index. C Chao1 index. D Ace index. E UPGMA clustering method. F PCA of gut microbiota based on the OTU data of the control, PCOS, and omega-3 groups. PCA, principal component analysis; UPGMA, unweighted pair group method using arithmetic average. The data are shown as the mean ± SEM with individual values. Statistical analyses were carried out using one-way ANOVA followed by Dunnett’s multiple comparisons test (*P < 0.05; **P < 0.01)