Table 1 Primer sequences for RT-qPCR (from 5’ to 3’ terminal)
Genes | Forward | Reverse |
|---|---|---|
β-actin | 5’-GGCTGTATTCCCCTCCATCG-3’ | 5’- CCAGTTGGTAACAATGCCATGT-3’ |
Ucp1 | 5’-AGGCTTCCAGTACCATTAGGT-3’ | 5’-CTGAGTGAGGCAAAGCTGATTT-3’ |
Pgc1a | 5’-TATGGAGTGACATAGAGTGTGCT-3’ | 5’-CCACTTCAATCCACCCAGAAAG-3’ |
Cited1 | 5’-AACCTTGGAGTGAAGGATCGC-3’ | 5’-GTAGGAGAGCCTATTGGAGATGT-3’ |
Cox8b | 5’-TGTGGGGATCTCAGCCATAGT-3’ | 5’-AGTGGGCTAAGACCCATCCTG-3’ |
Il1b | 5’- ATGATGGCTTATTACAGTGGCAA-3’ | 5’- GTCGGAGATTCGTAGCTGGA-3’ |
Il18 | 5’- TCTTCATTGACCAAGGAAATCGG-3’ | 5’- TCCGGGGTGCATTATCTCTAC-3’ |
Tnfa | 5’- GAGGCCAAGCCCTGGTATG-3’ | 5’- CGGGCCGATTGATCTCAGC-3’ |
Ccl2 | 5’- TTAAAAACCTGGATCGGAACCAA-3’ | 5’-GCATTAGCTTCAGATTTACGGGT-3’ |