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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: Mutations in CCNB3 affect its location thus causing a multiplicity of phenotypes in human oocytes maturation by aberrant CDK1 activity and APC/C activity at different stages

Fig. 3

Mimicking the oocyte maturation arrest phenotype in mouse oocytes in vitro, effects of CCNB3 mutation on protein level and location. (A) Images of mouse GV oocytes injected with WT or mutant cRNAs and cultured for up to 3 h. (B) Quantitative analysis of GVBD rate. (C) Images of mouse GV oocytes injected with WT or mutant cRNAs and cultured for up to 13 h. (D) Quantitative analysis of PB1 rate. Graphs show means ± SEM of results observed in 3 independent experiments. **P < 0.01, **** p < 0.0001. (E) The effects of the mutations on CCNB3 protein level by Western blot in transfected HEK-293 cells. (F) Quantitative analysis of CCNB3 protein level. (G) Predict the domains of the CCNB3 protein, the positions of E116A and E221A mutations are closed to NLS and the positions of P1223L and S1353N are close to NES. NES: nuclear export signal; NLS: nuclear localization signal. (H) Immunofluorescence results of oocytes injected with CCNB3 (WT or mutant) cRNA; Blue: DAPI; Green: flag-WT or flag-mutated CCNB3. The scale bar represents 50 μm. Data are shown as the means ± SEM. For each kind of variant,n = 10 biological replicates. (I) Relative intensity of cytoplasmic/nuclear representing foci intensity

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Journal of Ovarian Research

ISSN: 1757-2215

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