Fig. 6

Phenotypic rescue by using PP1 inhibitor or CDK1 inhibitor at different stages. (A-C) diagram depicting the flow of GV oocyte culture. GV oocytes were injected with wild-type (WT) or mutant CCNB3 cRNA into cytoplasm and subsequently treated with okadaic acid(2µM) and culture for 3 h, The percentages of GVBD were scored. (D-F) Images of mouse GV oocytes injected with WT or mutant cRNAs and cultured for up to 10 h, and then treated with rosvintine (0.2mM) and culture for 3 h, the percentages of MII were scored. The scale bar represents 80 μm. Data are shown as the means ± SEM. For each group,n = 3 biological replicates