Fig. 3

EEF2 protein interacted with lncRNA Gm2044. (A) The lncRNA Gm2044 can adsorb EEF2 protein. The control probe and Gm2044 probe were used to capture RNA-protein complex and then subjected to western blotting detecting EEF2 protein and β-Actin protein (negative control). (B) RNA IP revealed that EEF2 protein can capture lncRNA Gm2044 and bind Nr5a1 mRNA. IgG and anti-EEF2 antibodies were used to adsorb protein-RNA complex and then subjected to western blotting, detecting EEF2 protein and RT-PCR detected lncRNA Gm2044 and Nr5a1 mRNA.