Fig. 4

LncRNA Gm2044 knockout mice were successfully constructed. (A) The schematic diagram for exons 1 and 2 of lncRNA Gm2044 knockout using the CRISPR/Cas9 method. (B) The location of primers detecting genotype on lncRNA Gm2044 gene. (C) Genotyping of lncRNA Gm2044 for mice Genomic DNA was isolated from the mouse tail and then subjected to PCR using primer 1–4. Primer 1&2 got Gm2044 [+] fragment (2099 bp) and Gm2044 [-] fragment (531 bp). Primer 3&4 got Gm2044 [+] fragment (609 bp). Rgs7 was used as the reference gene. (D and E) There was no expression of lncRNA Gm2044 in knockout mice. Total RNA was extracted from the ovary and then subjected to qPCR (D) and RT-PCR (E). *, p < 0.05; ***, p < 0.001