Fig. 4
Exercise and myokine induction inhibit ROS production and improve follicular development in PCOS rats. Rats received DHEA to induce polycystic ovary syndrome followed by exercise treatment. A The RNA levels of PGC-1α, FNDC5, IL-6, IL-15, Angptl4, FGF-2, and myostatin were analyzed by qRT-PCR. B Serum irisin levels were analyzed using ELISA. C The expression of integrin αVβ5 in ovaries was analyzed by immunofluorescence staining (50 ×). Data are shown as the mean ± SD. *p ≤ 0.05, **p ≤ 0.01. DHEA, dehydroepiandrosterone; D + E, DHEA + Exercise; PGC-1α, peroxisome proliferator-activated receptor-γ coactivator-1α; FNDC5, fibronectin type III domain-containing protein-5; IL-6, interleukin-6; IL-15, interleukin-15; FGF-2, fibroblast growth factor 2; αVβ5, integrin αVβ5