Table 1 Primers of the genes used in the study
Genes | Forward | Reverse |
|---|---|---|
β-actin | 5’-TTCCTTCCTGGGTATGGAAT-3’ | 5’-GAGGAGCAATGATCTTGATC-3’ |
TGF-β | 5’-TACTGCTTCAGCTCCACAGAGA-3’ | 5’-CAGACAGAAGTTGGCATGGTAG-3’ |
α-SMA | 5’-AGGGACTAATGGTTGGAATGG-3’ | 5’-CAATCTCACGCTCACGCTCGGCAGTAG-3’ |
β-catenin | 5’-ACCATCGAGAGGGCTTGTTG-3’ | 5’-CGCACTGCCATTTTAGCTCC-3’ |
Fibronectin | 5’-TGACAACTGCCGTAGACCTGG-3’ | 5’-TACTGGTTGTAGGTGTGGCCG-3’ |
NLRP3 | 5’-CAGCGATCAACAGGCGAGAC-3’ | 5’-AGAGATATCCCAGCAAACCTATCCA-3’ |
ASC | 5’-GGACCAACACAGGCAAGCACTC-3’ | 5’-ACAAGTTCTTGCAGGTCAGGTTCC-3’ |
IL-1β | 5’-CTACCTATGTCTTGCCCGTGGAG-3’ | 5’-GGGAACATCACACACTAGCAGGTC-3’ |
TXNIP | 5’-AGATAGAGTATATCTTCAAGCCG-3’ | 5’-CTATGTGCTGGCTTTGGT-3’ |
GSDMD | 5’-TTGAGTGTCTGGTGCTCGAC-5’ | 5’-ATGGGGTGCTCTGTTCCAAG-5’ |
IRE1α | 5’-CCAACCACTCACTCAACTCT-3’ | 5’-TTTTCCCAACAATCACCA-3’ |
PGC-1α | 5’-ACATCGCAATTCTCCCTT-3’ | 5’-CTCTTGAGCCTTTCGTGCTC-3’ |
FNDC5 | 5’-TGGAGGAGGACACAGAGTATATCG-3’ | 5’-CATATCTTGCTTCGGAGGAGACC-3’ |
IL-6 | 5’-TATGAACAGCGATGATGCACTG-3’ | 5’-TTGCTCTGAATGACTCTGGCTT-3’ |
IL-15 | 5’-GACAGTGACTTTCATCCCAGTT-3’ | 5’-CATTCCTTGCAGCCAGAC-3’ |
Angptl4 | 5’-AGAAGTTGGAGATGCAGAGGGAC-3’ | 5’-CCACAAGAGCACCATTGAGTGTAT-3’ |
FGF-2 | 5’-CAGTGAGTGCCGACCCGCTC-3’ | 5’-GCGGGAAGACAGCCAGTCCG-3’ |
Myostatin | 5’-ATCTGAGAGCCGTCAAGACTCC-3’ | 5’-CAGTCAAGCCCAAAGTCTCTCC-3’ |
αVβ5 | 5’-TGCCAAGTTCCAAAGCG-3’ | 5’-GGTCCAAGGAGTCCGAGAC-3’ |