Fig. 2

Abnormal cell cycle distribution in Fance deficient PGCs during rapid mitosis. (A) Representative images of co-localized staining of EdU and SSEA1 in Fance^−/− PGCs and Fance^+/+ PGCs. (B) Representative images of co-localized staining of Cyclin B1 and SSEA1 in Fance^−/− PGCs and Fance^+/+ PGCs. (C)Fance^−/− mice had decreased proportion of PGCs in S and G2 phases and an increased proportion of PGCs in M phase compared to Fance^+/+ mice (S, EdU positive; G2, Cyclin B1 positive in cytoplasm; M, Cyclin B1 positive in nucleus). n = 4 embryos. All bars = 200 μm