Fig. 1

miR-338-3p expression in ovarian cortical tissues and granulosa cells. (A) Next-generation sequencing was performed to analyse miRNA-expression profile in the ovarian cortical tissues derived from patients with POI or normal ovarian function, and a clustering-analysis map was plotted for the differentially expressed miRNAs. The red and green blocks indicate differentially expressed genes in ovarian cortical tissues derived from POI patients that were up-regulated or down-regulated, respectively. The X-axis corresponds to the ovarian cortical tissue samples derived from patients with normal ovarian function or POI, and the Y-axis corresponds to the differentially expressed miRNAs. (B) Follicular fluid samples were collected from patients with normal ovarian function or a low ovarian reserve. The primary GCs were purified by density-gradient centrifugation and total RNA was extracted using TRIzol. After the extracted total RNA was reverse transcribed, real-time PCR was performed to detect the relative expression levels of miR-338-3p. U6 RNA was detected as the internal reference. ****P < 0.0001