Fig. 2

Expression and localization of miR-338-3p in human primary GCs and mouse ovaries. (A&B) Primary human GCs in human follicular fluid from patients with normal ovarian function (A) or DOR (B) were purified by density-gradient centrifugation, inoculated on cell slides in 6-well plates, and cultured for 24 h. After routine fixation of the cells, in situ hybridization was performed to detect the expression and localization of miR-338-3p. (C&D) After routine fixation and embedding of the obtained normal mouse ovaries (C) or POI mouse ovaries (D), in situ hybridization was performed to detect the expression and localization of miR-338-3p in the tissues. The blue signals reflect nuclear staining by DAPI. The red signals represent the NC or miR-338-3p signals, as indicated. Scale bars: 50 μm