Fig. 5

The effect of miR-338-3p overexpression on follicular development in neonatal mouse ovaries cultured in vitro. (A&B) After routine fixation and embedding of the obtained D1 mouse ovaries (A) or D4 mouse ovaries (B), in situ hybridization was performed to detect the expression and localization of miR-338-3p in the tissues. (a): fresh-control group. (b): adenovirus-control group. (c): miR-338-3p-overexpression group.The blue signals reflect nuclear staining by DAPI. The red signals represent the NC or miR-338-3p signals, as indicated. (C): (a, d) The fresh-control group consisted of neonatal (D1, D4) mouse ovaries that were obtained, immediately fixed, routinely embedded, and then HE-stained. (b, e) The adenovirus-control group consisted of neonatal (D1, D4) mouse ovaries that were cultured in vitro, treated with adv-EGFP for 96 h, and then fixed. (c, f) The miR-338-3p-overexpression group (i.e. the experimental group) consisted of neonatal (D1, D4) mouse ovaries that were cultured in vitro, treated with adv-miR-338-3p for 96 h to induce miR-338-3p overexpression, and then fixed. Scale bars: 50 μm