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Table 5 The qRT-PCR reaction system

From: A methylation- and immune-related lncRNA signature to predict ovarian cancer outcome and uncover mechanisms of chemoresistance

REAGENT

VOLUME

2xSuperReal Color PreMix

5 μl

Forward(10 μM)

0.3 μl

Reverse(10 μM)

0.3 μl

cDNA templet

2 μl

Rnase Free ddH2O

Fill in to 10 μl