Fig. 1

DHT in vivo treatment reduced NPY contents and Npy mRNA abundance in isolated granulosa cells. Granulosa cells of in vivo DHT treatment model rats were isolated. A, B Total protein was extracted and NPY contents were assessed by Western blot. Representative Western blot result is shown (A). C Total mRNA was extracted, reverse transcribed and amplified by real-time PCR using specific primers for Npy and Gapdh. Expression of Npy mRNA was normalized to RNA loading for each sample using Gapdh mRNA as an internal standard. DHT treatment significantly reduced Npy mRNA abundance and NPY content granulosa cells. These results indicate that hyperandrogenism reduces NPY expression in granulosa cells. One replicate of granulosa cells was isolated from one rat. Results are expressed as mean ± SEM of independent replicates (A, B; CTL, n = 4; DHT, n = 4) (C; CTL, n = 6; DHT, n = 4) and analyzed by Welch’s test (B) and unpaired t test (C). *, p < 0.05