Fig. 1

PAPSS1 expression in EOC tissues and cell lines and role in cisplatin resistance. A, B Expression of PAPSS1 mRNA (A) and protein (B) in the normal ovarian epithelium and ovarian cancer tissues (21 pairs of EOC and epithelial normal ovary tissues) analyzed by qRT-PCR (A) and Western blot (B). C, D The expression of PAPSS1 mRNA (C) and protein (D) in EOC and normal ovarian cell lines (HOSEpic cells, cisplatin-sensitive A2780 cells, and cisplatin-resistant SKOV3 cells) by qRT-PCR (C) and Western blot (D). E Representative images and its regional magnification of PAPSS1 IHC in ovarian cancer and normal ovarian epithelium tissues: high expression of PAPSS1 is seen in ovarian cancer tissues; low expression of PAPSS1 is seen in the nuclear of normal ovarian epithelium tissues. Scale bar: 200 µm and 50 µm. F Relative expression of PAPSS1 in ovarian cancer and epithelial normal ovary tissues by boxplot graph identified based on TCGA datasets (ovarian cancer n = 426 and normal ovarian tissues n = 88). Image data derived from TCGA Ovarian Cancer Database information, where N indicates P > 0.05 (not a statistically significant difference). The red is tumor tissue and grey is normal tissue. G PAPSS1 transcript expression is upregulated in metastatic tissues relative to the primary tumor and normal tissue. Data shown represent the mean ± SD. ^NP > 0.05; *P < 0.05. **P < 0.01, ***P < 0.001