Fig. 2
PAPSS1 regulates EOC cell proliferation and sensitivity to cisplatin in vitro. A Western blots show the relative PAPSS1 protein levels in untreated and cisplatin-sensitive A2780 cells and cisplatin-resistant SKOV3 cells. Densitometric analysis of the levels of PAPSS1 protein is shown at the bottom in A. The black column represents untreated cells, and the white column represents cisplatin treatment cells. B, C The transfection efficiency of PAPSS1 siRNA determined by qRT-PCR and Western blots. D, E The proliferation and the cisplatin IC50 of PAPSS1 silenced cells determined by CCK-8 assay. F Cells were treated with the indicated doses of cisplatin for 24 h, and colony formation assays were conducted to determine the cell survival ability for siPAPSS1 transfected EOC cells. Data shown represent the mean ± SD. *** P < 0.001,** P < 0.01