Fig. 3

PAPSS1 regulates EOC cell apoptosis and cell cycle. A, B A2780 and SKOV3 cells were treated with low doses of cisplatin (1 or 10 μM) for 24 h. Flow cytometry was performed to determine the apoptosis and cell cycle of PAPSS1 silenced cells (the black arrow points to the S phase block in the cell cycle of EOC cells). Densitometric analysis of the apoptotic rate and cell cycle are shown in the right side in A and B. C A2780 and SKOV3 cells were treated with low doses of cisplatin (1 or 10 μM) for 24 h, respectively. Cells were stained with Hoechst 33342. Highly condensed or fragmented nuclei represent apoptotic cells. Intact nuclei represent viable cells. The white arrows indicate early-stage apoptotic nuclei. Densitometric analysis of the apoptotic cell rate is shown at the bottom in C. Scale bar: 100 µm. D Apoptosis-related protein Bax, Bcl-2 and p-AKT levels were determined by Western blots. Images were visualized using the in Cell Analyzer 2200. Data shown represent the mean ± SD. ** P < 0.01