Fig. 4

PAPSS1 regulates EOC cell DNA damage. A For immunofluorescent staining, A2780 and SKOV3 cells were treated with low doses of cisplatin (1 or 10 μM) for 24 h, respectively. Cells stained with anti-γH2AX antibody (green) and counter-stained with DAPI (blue). The percentage of cells with more than one γH2AX-positive puncta are quantified and plotted at the bottom in A. Scale bar: 50 µm. The data shown represent the mean ± SD. ** P < 0.01,*** P < 0.001. B, C The expression of genes related to cell DNA repair (BRCA1, BRCA2) and drug transporters (MRP1, MRP2) in two siRNA cells compared to two si-NC cells examined by qRT-PCR. Western blot (C). The gene expression in fold-change was calculated by the delta-delta Ct method. The data shown represent the mean ± SD. Statistically significant (P < 0.01) changes in SKOV3 and A2780 cells are indicated by symbols ** and ^##, respectively