Fig. 4

Time-series analysis demonstrated different dynamic changes in gene expression between female and male samples. A Bar charts showing the correlated KEGG 2021 human pathways of the continuously upregulated (up-up-up) genes in female induced samples across the different timepoints in gonadal differentiation. The bar charts show the top 10 enriched terms in the chosen sample set, along with their corresponding p-values. Coloured bars correspond to terms with significant p-values (< 0.05). B Log2 plots showing the top 10 continuously upregulated genes (RP11-320G24.1, POMC, RP11-71L14.3, LINC00473, NOSTRIN, SGCZ, CCL22, RP11-491F9.2, NOV and MIR4486) in the female induced samples (IND F) compared with their expression in control female (CTRL F), induced male (IND M) and control male (CTRL M) samples. The asterisk (*) symbolizes significant expression of these genes