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Table 2 RT-qPCR primer pair sequences

From: CRISPR/Cas9-mediated activation of NR5A1 steers female human embryonic stem cell-derived bipotential gonadal-like cells towards a steroidogenic cell fate

Primer Name

Sequence of 5′ Primer (Forward)

Sequence of 3′ Primer (Reverse)

NR5A1

CAGGAGTTTGTCTGCCTCAA

GCACAGGGTGTAGTCAAGCA

GATA4

CAGGCGTTGCACAGATAGTG

CCCGACACCCCAATCTC

WT1

GGCAGCACAGTGTGTGAACT

CCAGGCACACCTGGTAGTTT

FOXL2

TTTGTCCCCTCAGTTTATGTCC

TGAATTTGGGCAGGAGACG

WNT4

GATGTGCGGGAGAGAAGCAA

ATTCCACCCGCATGTGTGT

RSPO1

GCAACCCCGACATGAACAAG

CAAGCCCTCCTTACACTTGG

STAR

GAGTCAGCAGGACAATGGGG

CGCTCCACGAGCTCTTCATA

CYP11A1

TGGTGACAATGGCTGGCTAAA

ATAAACCGACTCCACGTTGC

CYP17A1

TCAGCCGCACACCAACTATC

GCAAACTCACCGATGCTGGA

CYP19A1

GTGGCCCATGGCATTTTATA

GAGCTCTACTGGGGAACCAG

HPGD

CAGCCGGTTTATTGTGCTTC

CAGTCTCACACCACTGTTCATA

HSD3B2

GAGGCAGTAAGGACTTGGACT

TGACCCAGAAGAGGGCGTAA

HSD17B1

TGATGGGGCTGCCTTTCAAT

ATCAGGCTCAAGCTGTGGAC

GSTA1

GATGTTCCAGCAAGTGCCAAT

CAGGTGGACATACGGGCA

GSTA2

ATCCTTCTTCTGCCCTTTAGTC

GTCTTGTCCGTGGCTCTTTA

WNT6

AACAGGACATTCGGGAGACG

CAGCTCGCCCATAGAACAGG

B4GALNT2

TACCAAGACTTTCCTCCGCC

AGCAAACCAACCCTTCCCAA

GNRHR

AGTTTTTCACAATGGTGGCATCAA

TGGCAAATGCAACCGTCATT

PPIG

TCTTGTCAATGGCCAACAGAG

GCCCATCTAAATGAGGAGTTG