Fig. 3
Construction of gene co-expression networks associated with POI through WGCNA. A Determination of the soft-thresholding power (β). The left panel represents the analysis of the scale-free fit index for different soft-thresholding powers, and the right panel represent the analysis of the mean connectivity for different soft-thresholding powers. B Histogram of connectivity distribution when β = 7 and determining the scale-free topology when β = 7. C The cluster dendrogram and hierarchical clustering heatmap of module eigengenes based on the dissimilarity of TOM. Each branch represents one gene and each color represents one co-expressed module. D The heatmap plot represents interactive relationship of different module genes. E The heatmap of correlation between genes in different modules and the clinical traits. F Scatter plot of GS and MM for group in brown module. WGCNA: weighted gene co-expression network analysis; TOM: topological overlap measure; GS: gene significance; MM: module membership; FSH: follicle-stimulating hormone; AMH: anti-Mullerian hormone; LH: luteinizing hormone; E2: estradiol; PR: progesterone; AFC: antral follicle counts; BMI: body mass index