Fig. 7

UBE2C Knockdown induced apoptosis of GCs. A Flow cytometry analysis showed the apoptosis rate of KGN was significantly increased after depletion of UBE2C. Data were presented as the means ± SEM from three repeated experiments. B TUNEL analysis showed that the proportion of TUNEL positive cells was upregulated significantly after knockdown of UBE2C. The apoptotic cells were stained with TUNEL (red) and all cells were stained with DAPI (blue). Scale bar = 100 um. Values were presented as the means ± SEM from three repeated experiments. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, two-tailed Student’s t-test)