Fig. 4

Critical factors boost EMT in EOC. A Up- and downregulated TFs and their Pearson correlation coefficients with pseudotime. B Transcriptional regulatory network constructed using temporal-associated genes. The diamond represents the TF, and the circle represents the target gene. The size of the graph is determined by the coefficients of positively (yellow) and negatively (green) genes. C-E Survival curves of FOSB, RUNX1 and EGR1 gene expression in the TCGA-OV dataset. F Schematic diagram of the reversal of epithelial cells with reduced E-cadherin into mesenchymal cells. G-J Variation in the expression levels of FOSB, RUNX1, EGR1, and HSPG2, which are transcription factors and target genes, with pseudotime. K Box plot of gene expression variations, including FOSB, RUNX1, EGR1, and HSPG2, between iE and iM samples of bulk RNA-seq from TCGA