Fig. 3

Expression of wild-type and mutant TUBB8 proteins in HeLa cells. a,b The protein expression level of TUBB8 in HeLa cells transfected with plasmids encoding Myc-tagged wild-type and mutant TUBB8 proteins. WT, wild-type. * P < 0.05, ** P < 0.01, *** P < 0.001. c,d Microtubule phenotypes induced by the expression of wild-type and mutant TUBB8 proteins in HeLa cells. Cells were immunoassayed with an antibody against Myc epitope (green) to visualize transgene and counterstained with an antibody against α-TUBULIN (red) to visualize endogenous microtubule network. Scale bar, 10 μm. WT, wild-type. * P < 0.05, ** P < 0.01, *** P < 0.001