Fig. 4

Phenotypes of oocytes and embryos from probands withTUBB8variants. a Morphology of oocytes from a normal control and three probands on day 0. The black arrows indicate polar bodies. Scale bar, 50 μm. b Morphology of zygotes on day 1, and embryos on day 2 and day 3 from a normal control and four probands. The black stars indicate pronucleus. Scale bar, 50 μm. c β-TUBULIN/DNA immunostaining of a normal MI oocyte and two MI oocytes form Family 9. Oocytes were immunoassayed with an antibody against β-TUBULIN (green) to visualize spindle and counterstained with DAPI (blue) to visualize DNA.