Fig. 5

hUC-MSCs ameliorated excessive autophagy of GCs via paracrine in vitro. A Schematic diagram of 3D MSCs spheroids fabrication with KGN/GCs co-culturing. The autophagy-related proteins (P62, Beclin-1, ATG5, LC3A/B) and ovarian GCs function proteins (FSHR and Cyp19a1) expressions in KGN (B) and primary GCs (C), co-cultured with 3D MSCs spheroids. The autophagy protein LC3B (D) and proliferation protein Ki67 (E) expressions in KGN and primary GCs after co-culturing with 3D MSCs spheroids using immunofluorescence detection; scale bar: 100 μm in KGN and 50 μm in GCs. ROS (F), GSH (G), and MDA (H) levels in KGN and primary GCs after co-culturing with 3D MSCs spheroids. *p < 0.05. **p < 0.01