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Fig. 8 | Journal of Ovarian Research

Fig. 8

From: Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) alleviate excessive autophagy of ovarian granular cells through VEGFA/PI3K/AKT/mTOR pathway in premature ovarian failure rat model

Fig. 8

VEGFA improved excessive autophagy of GCs via PI3K/AKT/mTOR in ovarian culture in vitro. A The histologic features of the ovary in CTX damaging three-day-old rats rescued by VEGFA; scale bar: 100 μm. B The number of primordial and primary follicles in ovaries of Control, CTX, and CTX + VEGFA groups. C The autophagy proteins (P62, Beclin-1, and LC3A/B) and marker protein (Cyp19a1) expressions in GCs in ovaries among Control, CTX, and CTX + VEGFA groups. D Cyp19a1 and Ki67 expressions in ovaries among Control, CTX, and CTX + VEGFA groups by immunofluorescence detection; scale bar: 50 μm. E The effects of follicle development related genes Lhx8, Nanos3, Lin28a, Nobox and Bmp15 in transcriptional level among Control, CTX and CTX + VEGFA groups. F The autophagy protein LC3B expression in ovaries among Control, CTX, and CTX + VEGFA groups via immunofluorescence detection; scale bar: 50 μm. G The levels of lipid peroxidation products 4-HNE in ovaries among Control, CTX, and CTX + VEGFA groups; scale bar: 50 μm. The oxidative stress markers GSH (H) and MDA (I) levels in ovaries among Control, CTX, and CTX + VEGFA groups. *p < 0.05. **p < 0.01

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Journal of Ovarian Research

ISSN: 1757-2215

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