Fig. 2
Characterization of EGF-stimulation. EGFR-signaling evaluation by (A) antibody-array (CAOV3 cells) and, (B) Western Blotting (OvCa cells) kept FBS-free for 18 h, followed by 15/30 minutes EGF-stimulation, showing MEK 1/2 total, phosphorylated MEK (Ser-217/Ser-221), p-44/42 MAPK (Erk 1/2) total, and phosphorylated p-44/42 MAPK (Erk 1/2) (Thr-202/Tyr-204). (C) evaluation of ROS (CM-H2DCFDA) and, (D) mitochondrial membrane potential (JC-1 aggregate/JC-1) of OvCa cells after EGF EMT-induction (mean ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001)