Fig. 3
Characterization of exosomes induced by EGF-induced EMT. (A) Western blot analysis of exosome markers. (B) Evaluation of size and concentration using nanoparticle tracking analysis (NTA), revealing a mean size of 140.5 nm and a concentration of 1.24 × 109/mL for the control (CT), and a mean size of 147.5 nm and a concentration of 2.18 × 109/mL for EGF-induced EMT (EGF 10ng/mL). (C) Transmission electron microscopy (TEM) characterization of exosomes isolated from CAOV3 cells under control conditions (CT) and after 96 h of EGF-induced EMT (EGF 10ng/mL)