Fig. 5
Simvastatin influences cell proliferation, migration, invasion, apoptosis and metabolism in OVE cells. OVE cells (n = 3/group) were treated with 10uM simvastatin or DMSO for all experiments. A OVE cells were treated for 24 h, brightfield images were captured using an IncuCyte S3 Live-Cell Analysis System and the percent confluency at each timepoint was calculated. B OVE cells were treated for 24 h then subjected to the Caspase-Glo 3/7 apoptotic assay and normalized to CyQUANT proliferative assay values. C OVE4 (wild-type, p53R175H mutant and Trp53 knockout) cells were treated for 24 h, then seeded and incubated for 24 h in transwell chambers. Cells were stained with toluidine blue and the percent area invaded was calculated. D OVE4 (wild-type, p53R175H mutant and Trp53 knockout) cells were treated for 3–48 h and subjected to a resazurin sodium salt cell metabolic assay. Bars represent mean ± SEM, (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Scale bars: 500 μm. Abbreviations: WT wild-type; MUT p53R175H mutant; KO Trp53 knockout