Fig. 6

Simvastatin inhibits ovarian cancer disease progression, increases apoptosis and HMGCR cytoplasmic localization, but does not influence YAP or small GTPase expression. A Mice were subjected to daily intraperitoneal injections of simvastatin (1 mg/kg) or PBS for 25 days, then tumors were collected and weighed (n = 8–9/group). B TUNEL staining (n = 6/group) was performed on PBS and simvastatin treated tumors. C Cytoplasmic and nuclear protein fractions were collected from flash-frozen PBS and simvastatin treated tumors (n = 3/group) and western blotting was performed for HMGCR and YAP. D PBS and simvastatin treated tumors (n = 6/group) were immunohistochemically stained for the small GTPases, Rho and Rac1. Bars represent mean ± SEM (**p < 0.01, ***p < 0.001). Scale bars: 100 μm. Abbreviations: C cytoplasmic; N nuclear