Fig. 6

PBM NPs downregulate an MDR gene in OvCa cells. Expression of ABCG2 protein in [A] OVCAR3 and [B] CAOV3 cells treated with Fis-FA- or PTX-FA-NPs, alone or in combination for 48 h. As an internal standard for equal loading, blots were probed with a GAPDH antibody. The band intensity of ABCG2 was normalized against GAPDH, and the densitometry is shown below. mRNA expression levels of the drug resistance gene were quantified by RT-PCR upon treatment and compared to the control in both [C] OVCAR3 and [D] CAOV3 cell lines. The expression level of ABCG2 is represented by fold change relative to the control. Data were normalized to the housekeeping gene levels, 18 S expression. Data are presented as the means ± standard error of the mean, and asterisks indicate significance determined by student t-test (*P < 0.05) (**P < 0.01)